PCGF1

Protein-coding gene in the species Homo sapiens
PCGF1
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

4HPL, 4HPM

Identifiers
AliasesPCGF1, 2010002K04Rik, NSPC1, RNF3A-2, RNF68, polycomb group ring finger 1
External IDsOMIM: 610231; MGI: 1917087; HomoloGene: 13090; GeneCards: PCGF1; OMA:PCGF1 - orthologs
Gene location (Human)
Chromosome 2 (human)
Chr.Chromosome 2 (human)[1]
Chromosome 2 (human)
Genomic location for PCGF1
Genomic location for PCGF1
Band2p13.1Start74,505,043 bp[1]
End74,507,695 bp[1]
Gene location (Mouse)
Chromosome 6 (mouse)
Chr.Chromosome 6 (mouse)[2]
Chromosome 6 (mouse)
Genomic location for PCGF1
Genomic location for PCGF1
Band6 C3|6 35.94 cMStart83,054,850 bp[2]
End83,057,836 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • oocyte

  • secondary oocyte

  • right lobe of liver

  • pituitary gland

  • body of stomach

  • body of pancreas

  • right uterine tube

  • anterior pituitary

  • monocyte

  • left lobe of thyroid gland
Top expressed in
  • secondary oocyte

  • adrenal gland

  • bone marrow

  • morula

  • olfactory bulb

  • pancreas

  • neural tube

  • islet of Langerhans

  • testicle

  • cerebellum
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • protein C-terminus binding
  • protein binding
  • metal ion binding
  • promoter-specific chromatin binding
Cellular component
  • PcG protein complex
  • nucleus
  • nucleoplasm
  • PRC1 complex
Biological process
  • histone H2A monoubiquitination
  • transcription, DNA-templated
  • regulation of transcription, DNA-templated
  • histone H2A-K119 monoubiquitination
  • negative regulation of transcription by RNA polymerase II
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

84759

69837

Ensembl

ENSG00000115289

ENSMUSG00000069678

UniProt

Q9BSM1

Q8R023

RefSeq (mRNA)

NM_032673

NM_197992

RefSeq (protein)

NP_116062

NP_932109
NP_001390487
NP_001390488
NP_001390489
NP_001390490

Location (UCSC)Chr 2: 74.51 – 74.51 MbChr 6: 83.05 – 83.06 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Polycomb group RING finger protein 1, PCGF1, also known as NSPC1 or RNF68 is a RING finger domain protein that in humans is encoded by the PCGF1 gene.[5][6]

PCGF1 is a component defining the non-canonical polycomb repressive complex 1.1 (ncPRC1) interacting with RING1A/B, RYBP, BCOR and KDM2B. [7] PCGF1-BCOR assembles via the ubiquitin-like RAWUL domain of PCGF1 and is recruited on the chromatin at KDM2B sites.[8][9][10] Within the PRC1-like complex, PCGF1 regulates RING1B ubiquitin ligase activity that catalyzes the ubiquitination of Lys119 on histone H2A, [11] which then leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain and mediate gene repression. [12][13][14]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000115289 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000069678 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Nunes M, Blanc I, Maes J, Fellous M, Robert B, McElreavey K (April 2001). "NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-derived structures of the peripheral nervous system". Mechanisms of Development. 102 (1–2): 219–22. doi:10.1016/S0925-4773(01)00288-X. PMID 11287196. S2CID 8344430.
  6. ^ "Entrez Gene: PCGF1 polycomb group ring finger 1".
  7. ^ Gao Z, Zhang J, Bonasio R, Strino F, Sawai A, Parisi F, et al. (February 2012). "PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes". Molecular Cell. 45 (3): 344–56. doi:10.1016/j.molcel.2012.01.002. PMC 3293217. PMID 22325352.
  8. ^ Wong SJ, Gearhart MD, Taylor AB, Nanyes DR, Ha DJ, Robinson AK, et al. (October 2016). "KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1". Structure. 24 (10): 1795–1801. doi:10.1016/j.str.2016.07.011. PMC 5088048. PMID 27568929.
  9. ^ Junco SE, Wang R, Gaipa JC, Taylor AB, Schirf V, Gearhart MD, et al. (April 2013). "Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs". Structure. 21 (4): 665–71. doi:10.1016/j.str.2013.02.013. PMC 4003909. PMID 23523425.
  10. ^ Farcas AM, Blackledge NP, Sudbery I, Long HK, McGouran JF, Rose NR, et al. (December 2012). Struhl K (ed.). "KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands". eLife. 1: e00205. doi:10.7554/eLife.00205. PMC 3524939. PMID 23256043.
  11. ^ Taherbhoy AM, Huang OW, Cochran AG (July 2015). "BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase". Nature Communications. 6: 7621. Bibcode:2015NatCo...6.7621T. doi:10.1038/ncomms8621. PMID 26151332.
  12. ^ Tamburri S, Lavarone E, Fernández-Pérez D, Conway E, Zanotti M, Manganaro D, Pasini D (February 2020). "Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression". Molecular Cell. 77 (4): 840–856.e5. doi:10.1016/j.molcel.2019.11.021. PMC 7033561. PMID 31883952.
  13. ^ Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, et al. (June 2019). "Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression". Molecular Cell. 74 (5): 1020–1036.e8. doi:10.1016/j.molcel.2019.03.024. PMC 6561741. PMID 31029541.
  14. ^ Blackledge NP, Farcas AM, Kondo T, King HW, McGouran JF, Hanssen LL, et al. (June 2014). "Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation". Cell. 157 (6): 1445–1459. doi:10.1016/j.cell.2014.05.004. PMC 4048464. PMID 24856970.

Further reading

  • Valk-Lingbeek ME, Bruggeman SW, van Lohuizen M (August 2004). "Stem cells and cancer; the polycomb connection". Cell. 118 (4): 409–18. doi:10.1016/j.cell.2004.08.005. PMID 15315754. S2CID 16542938.
  • Gong Y, Yue J, Wu X, Wang X, Wen J, Lu L, et al. (2007). "NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element". Nucleic Acids Research. 34 (21): 6158–69. doi:10.1093/nar/gkl834. PMC 1693893. PMID 17088287.
  • Gearhart MD, Corcoran CM, Wamstad JA, Bardwell VJ (September 2006). "Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets". Molecular and Cellular Biology. 26 (18): 6880–9. doi:10.1128/MCB.00630-06. PMC 1592854. PMID 16943429.
  • Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • Gong Y, Wang X, Liu J, Shi L, Yin B, Peng X, et al. (January 2005). "NSPc1, a mainly nuclear localized protein of novel PcG family members, has a transcription repression activity related to its PKC phosphorylation site at S183". FEBS Letters. 579 (1): 115–21. doi:10.1016/j.febslet.2004.11.056. PMID 15620699. S2CID 28187618.
  • Sugishita H, Kondo T, Ito S, et al. (September 2021). "Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes". Nat Commun. 12 (5341): 5341. Bibcode:2021NatCo..12.5341S. doi:10.1038/s41467-021-24894-z. PMC 8429492. PMID 34504070.


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